A complete listing of support material can be found on the 10x Genomics Support Page. The 10X Genomics Chromium Single-Cell System is used to provide a single-cell expression profiling technology that allows for high-throughput single cell transcriptomics of many different cell types as well as single-nuclei expression profiling. The 10x Genomic Resource page provides access to product literature, app notes, and posters.īelow are links highlighting demonstrated protocols and technical notes for specific applications. 10X Genomics Chromium Single-Cell System. ** 1 bp shorter to accommodate NovaSeq 100 cycle kits * minimum read count recommended by 10x Genomics increased sequencing depth may be desired for some studies Single Cell 5' RNA-Seq and V(D)J Profiling Bel-Art FlowmiTM Cell Strainer, 40 micron (Cat. Filtering cell suspensions is recommended to remove debris and clumped cells.Cell viability should be >70% with >90% being optimal.Strongly recommended that buffer is purchased from following vendors. It is best practice to provide periodic updates to core staff on the day of collection.ġx PBS with 0.04% BSA (calcium and magnesium free) On scheduled day of submission, core staff should be notified 1 hour prior to cells/nuclei being delived.Must minimize presence of cellualar aggregates, dead cell, non-cellular nucleic acids and reverse transcription inhibitors.Submit cells in Eppendorf DNA Lo Bind Tubes, 0.2ml (Cat.A Project Information Form should be completed and submitted by email prior to sample submission.Human and Animal - Use the BioSafety Form (Human and Animal).Along with the PAF, a biosafety form must also be submitted.The form must be signed and returned before services are started. The core director will prepare a Project Authorization Form(PAF) outlining the services.Number of sequence reads per cell/nuclei to be generated.Number cell/nuclei to be targeted for collection. The 10x Genomics Chromium is a microfluidics instrument that partitions single cells into nanoliter emulsions, allowing isolation of nucleic acids from. Answer: The Chromium Connect is a system that combines proprietary 10x microfluidics technology using the Chromium Automated Controller with precise, automated liquid handling that allows users to run 10x Genomics end to end library construction workflow with minimal user interaction.Several parameters are needed to quote a project and establish the scope of the work to be performed. Contact the core director to discuss project details.Projects should be initiated at least 2 weeks prior to anticipated collection of cells/nuclei to ensure there are no issues with scheduling of an experiment. Lunch provided to registrants.Prior to sample submission and services performed, a project must be initiated with the core director and involves the following steps. Learn how to enhance your biological discoveries with our genomics and high-throughput single cell transcriptomics products and explore our newest single cell technologies such as the Single Cell ATAC Solution and Single Cell Gene Expression Solution with Feature Barcoding technology. These are just a few of the ways our solutions can provide unparalleled insight into previously inaccessible information. Characterize and profile gene expression in hundreds to tens of thousands of single cells, sequence paired, full-length B-cell or T-cell repertoires, or profile hundreds to thousands of single cell genomes to reveal genome heterogeneity and understand clonal evolution. Whether you want to dissect cell-type differences, investigate the adaptive immune system, or discover copy number variation and genomic heterogeneity on a cell-by-cell basis, the Chromium System from 10x Genomics is the answer.
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